FastQCFastQC Report
Thu 2 Jun 2016
DRR015165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13801128
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292990.21229424145620562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261300.1893323502252859No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT174860.12669978859699005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151980.11012143355238789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA291300.058.3962861
GTATCAA553250.041.4295851
GTGGTAT109650.033.0954671
TGGTATC109100.032.0366742
TATCAAC717250.031.8335062
ATCAACG718600.031.645343
TCAACGC724750.031.3720114
CAACGCA763900.029.7046265
AACGCAG802600.028.3312686
GTACATG738400.025.0353221
TACATGG728000.024.7306672
ACGCAGA920650.024.6826027
CGCAGAG918300.024.6463978
GCAGAGT974100.022.8095639
ACATGGG753900.022.6619343
CATGGGT154900.020.4152454
GAGTACT496200.020.08189212-13
CATGGGG358700.019.9534744
CAGAGTA931550.019.8340710-11
AGAGTAC882000.019.09623310-11
Produced by FastQC (version 0.11.5)