FastQCFastQC Report
Thu 2 Jun 2016
DRR015164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17684323
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244950.13851251190107758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205740.11634033149021313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA291350.051.6433531
GTATCAA525350.039.7207261
ATCAACG653750.031.584223
TCAACGC658900.031.3430214
TATCAAC671100.030.8966082
GTGGTAT128550.028.9960231
CAACGCA716900.028.8118825
AACGCAG751050.027.596736
GTACATG701400.026.0912511
TACATGG693650.025.4503462
TGGTATC137750.025.3076292
ACGCAGA840100.024.6484367
CGCAGAG849400.024.3569978
ACATGGG717800.023.3259533
GCAGAGT901900.022.4900449
CATGGGG416850.020.120624
CAGAGTA854050.019.3790110-11
GAGTACA553100.018.6458781
AGAGTAC811350.018.44786510-11
GAGTACT482100.018.37252812-13
Produced by FastQC (version 0.11.5)