FastQCFastQC Report
Thu 2 Jun 2016
DRR015164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17684323
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230050.13008697025043028No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT215120.12164446442196289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205230.11605194046727149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA289000.052.285751
GTATCAA518600.038.8906441
ATCAACG646800.030.7804723
TCAACGC654850.030.4034634
TATCAAC668950.029.9112072
CAACGCA697700.028.5362035
GTGGTAT129550.027.5871091
AACGCAG735000.027.1594356
GTACATG705350.025.6183011
TGGTATC137150.025.28312
TACATGG695900.024.9339522
ACGCAGA846200.023.5624337
CGCAGAG849350.023.4161838
ACATGGG733350.022.4576993
GCAGAGT906500.021.5584419
CATGGGG415450.019.1868174
GAGTACA569050.018.9454631
CAGAGTA858050.018.84394610-11
GAGTACT470450.018.31226512-13
AGTACAT545750.018.177492
Produced by FastQC (version 0.11.5)