FastQCFastQC Report
Thu 2 Jun 2016
DRR015163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19519772
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257340.13183555627596472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215970.11064166118333758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA312750.053.38931
GTATCAA582000.040.87271
ATCAACG742550.031.7097113
TCAACGC743350.031.6622054
TATCAAC764300.030.914672
GTGGTAT129450.029.601511
CAACGCA796200.029.5590975
AACGCAG831600.028.3325526
TGGTATC138600.026.037772
GTACATG855950.025.3082661
ACGCAGA934400.025.170827
TACATGG847100.024.6265582
CGCAGAG955850.024.5819118
ACATGGG869150.022.7764533
GCAGAGT1019350.022.5729779
CATGGGG483200.019.438324
CAGAGTA963850.019.32350510-11
CATGGGT203950.019.278184
AGAGTAC918500.018.84881810-11
GAGTACA673500.018.3390181
Produced by FastQC (version 0.11.5)