FastQCFastQC Report
Thu 2 Jun 2016
DRR015163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19519772
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251070.1286234285933258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218760.11207098115695205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA315850.053.6875231
GTATCAA590150.040.9555781
ATCAACG754400.031.698823
TCAACGC756600.031.5654434
TATCAAC774250.031.0540282
CAACGCA800150.029.8818455
GTGGTAT130450.029.390881
AACGCAG839700.028.4941676
TGGTATC140700.025.7152982
ACGCAGA954950.025.0259327
GTACATG871500.024.870481
CGCAGAG974550.024.4990188
TACATGG865300.024.0994382
GCAGAGT1049550.022.371159
ACATGGG888300.022.2136123
CAGAGTA988550.019.14206110-11
CATGGGG495200.018.9180934
AGAGTAC939750.018.77301810-11
CATGGGT207050.018.3709964
GAGTACT514650.018.05797212-13
Produced by FastQC (version 0.11.5)