FastQCFastQC Report
Thu 2 Jun 2016
DRR015162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11699626
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242960.2076647578307204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212460.1815955484388988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125570.10732821715839463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA240300.056.2298971
GTATCAA438300.039.5399671
GTGGTAT86400.032.1838721
ATCAACG548750.031.2317433
TCAACGC559000.030.666424
TATCAAC561550.030.6581362
TGGTATC89850.029.8835222
CAACGCA603400.028.3858095
AACGCAG648800.026.4263136
CGCAGAG698250.024.4626278
GTACATG511400.024.401561
TACATGG502700.024.1169932
ACGCAGA713500.024.0164057
GCAGAGT737850.022.7302049
ACATGGG528100.021.9742683
CAGAGTA709200.020.07592610-11
GAGTACT408650.019.69999112-13
CATGGGT93100.019.6496874
CATGGGG260050.019.5292974
GTACTTT404200.019.10739314-15
Produced by FastQC (version 0.11.5)