FastQCFastQC Report
Thu 2 Jun 2016
DRR015162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11699626
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252720.21600690483610333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225970.19314292610721062No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT196970.16835580898056057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137450.11748238789855334No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT127990.10939665934620474TruSeq Adapter, Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251650.054.8986171
GTATCAA462450.039.720421
GTGGTAT94000.034.344811
TGGTATC100050.031.204122
ATCAACG585050.031.1768973
TATCAAC592650.030.9772262
TCAACGC591150.030.8403224
CAACGCA628900.028.9674155
AACGCAG677150.027.0546466
GTACATG537200.024.8355831
CGCAGAG741800.024.481328
TACATGG529900.024.4596942
ACGCAGA756450.024.181647
GCAGAGT785050.022.8022149
ACATGGG546450.022.6385483
CATGGGT100250.019.9240384
GAGTACT439700.019.83211712-13
CAGAGTA753750.019.81019410-11
CATGGGG268450.019.5417354
GTACTTT430950.019.32217214-15
Produced by FastQC (version 0.11.5)