FastQCFastQC Report
Thu 2 Jun 2016
DRR015161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470282
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266480.23232209984026547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241200.21028253708147718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138080.12038064975211595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249200.058.1689531
GTATCAA458000.040.0270731
GTGGTAT90450.034.56831
TGGTATC91100.033.271212
ATCAACG585750.031.0236033
TCAACGC593750.030.6039984
TATCAAC598150.030.5559922
CAACGCA634900.028.6403275
AACGCAG666600.027.37266
GTACATG569150.024.7253571
CGCAGAG740750.024.552078
ACGCAGA744050.024.4799777
TACATGG558650.024.3988762
GCAGAGT788950.022.7403559
ACATGGG584200.022.37323
CATGGGT171500.021.3606384
CAGAGTA761750.020.30577310-11
GAGTACT438550.020.18658812-13
GTACTTT445950.019.25044414-15
AGAGTAC726950.019.08466510-11
Produced by FastQC (version 0.11.5)