FastQCFastQC Report
Thu 2 Jun 2016
DRR015161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470282
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259160.2259403910034644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241850.21084921887709474No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT203380.1773103747580051No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG142850.12453922231380188RNA PCR Primer, Index 27 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137160.1195785770567803No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT128610.11212453189904135RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA253950.057.3549771
GTATCAA474400.040.5496521
GTGGTAT94350.035.2278441
TGGTATC96400.033.5646132
ATCAACG602450.031.6511213
TCAACGC611900.031.1859784
TATCAAC615750.031.0778272
CAACGCA646300.029.5053375
AACGCAG678050.028.1396146
GTACATG575450.025.2161961
ACGCAGA768750.024.8242157
TACATGG566500.024.822622
CGCAGAG768900.024.7775178
GCAGAGT816550.023.0195649
ACATGGG593050.022.8775123
CATGGGT177100.021.169964
CAGAGTA784750.020.12011310-11
GAGTACT450300.019.93505312-13
CATGGGG251100.019.7510954
CATGGGA189900.019.2398624
Produced by FastQC (version 0.11.5)