FastQCFastQC Report
Thu 2 Jun 2016
DRR015160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14669400
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250650.17086588408523867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214410.14616139719415927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267300.055.796651
GTATCAA505800.040.5815241
ATCAACG634550.031.9655553
TCAACGC639250.031.7855324
TATCAAC645850.031.639242
CAACGCA685850.029.6306845
GTGGTAT112550.028.7915551
AACGCAG726800.028.0473276
TGGTATC112800.027.6371252
GTACATG659400.026.1589871
TACATGG647300.025.8382512
CGCAGAG807550.025.0785968
ACGCAGA812350.025.0368337
ACATGGG663250.023.9543
GCAGAGT863700.022.9687399
CATGGGT193500.022.2714144
CATGGGG329050.020.6479384
CAGAGTA829650.019.6805110-11
GAGTACT461800.019.49041212-13
GAGTACA515950.019.071231
Produced by FastQC (version 0.11.5)