FastQCFastQC Report
Thu 2 Jun 2016
DRR015160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14669400
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251870.17169754727528053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225230.1535372953222354No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT182700.1245449711644648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271400.055.5977021
GTATCAA509800.041.030361
ATCAACG653450.031.6295813
GTGGTAT110100.031.5951371
TCAACGC661000.031.3037874
TATCAAC664200.031.2823492
TGGTATC110800.029.9809512
CAACGCA699250.029.5588915
AACGCAG735750.028.1457126
GTACATG669600.025.7713931
TACATGG655200.025.398812
ACGCAGA842150.024.5620867
CGCAGAG837750.024.5608228
ACATGGG673550.023.5934733
CATGGGT204000.023.1050784
GCAGAGT895350.022.6148629
GAGTACT478800.019.6228612-13
CAGAGTA856050.019.40107210-11
CATGGGG331600.019.3456134
AGAGTAC809000.019.03609810-11
Produced by FastQC (version 0.11.5)