FastQCFastQC Report
Thu 2 Jun 2016
DRR015159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14199934
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216230.15227535564601918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179810.12662734911303108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238150.054.6518751
GTATCAA447000.039.766221
ATCAACG560250.031.4185813
TCAACGC569550.030.9609414
TATCAAC574350.030.7579822
CAACGCA623550.028.3071925
GTGGTAT95550.028.0526711
AACGCAG646450.027.282356
GTACATG626550.026.7652551
TACATGG619600.026.073292
TGGTATC102200.025.0689662
ACGCAGA707500.024.8949537
CGCAGAG723700.024.28738
ACATGGG646600.023.8656923
GCAGAGT779550.022.179479
CATGGGG358400.020.9883924
CATGGGA208950.019.709724
CAGAGTA743550.019.30504610-11
CATGGGT113500.018.763954
AGAGTAC712050.018.7599810-11
Produced by FastQC (version 0.11.5)