FastQCFastQC Report
Thu 2 Jun 2016
DRR015159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14199934
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226270.1593458110439105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193840.1365076767258214No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT157420.11085967019283328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251900.055.5223351
GTATCAA472900.039.5971641
ATCAACG598350.030.9324363
TCAACGC605350.030.5975114
TATCAAC607900.030.5503562
GTGGTAT101350.028.8843021
CAACGCA645350.028.7152235
AACGCAG668200.027.7705386
TGGTATC107100.025.9617252
GTACATG630250.025.7959581
TACATGG624350.025.1316452
ACGCAGA748450.024.7493347
CGCAGAG765050.024.1644048
ACATGGG650150.023.030473
GCAGAGT819900.022.1982239
CATGGGG353850.020.6370374
CAGAGTA785050.019.43417210-11
AGAGTAC748250.018.81800310-11
CATGGGA213250.018.711224
GAGTACA496900.018.5720311
Produced by FastQC (version 0.11.5)