FastQCFastQC Report
Thu 2 Jun 2016
DRR015158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16110450
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328510.20391112600827416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271060.1682510420255176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166080.10308836810889825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303950.058.528751
GTATCAA586900.042.082491
GTGGTAT119800.032.2140121
TATCAAC767800.031.9885982
ATCAACG768850.031.814993
TCAACGC780100.031.3610384
TGGTATC119600.031.3535652
CAACGCA834150.029.2887635
AACGCAG880000.027.879956
GTACATG879100.026.930091
TACATGG866200.026.4948642
CGCAGAG986100.024.7228458
ACGCAGA997400.024.5614347
ACATGGG885350.024.5393123
GCAGAGT1054100.022.7999179
CATGGGA417150.021.5454864
CATGGGT150850.021.1170964
CATGGGG361450.020.887174
GAGTACT529850.020.01711312-13
CAGAGTA1015650.019.55139710-11
Produced by FastQC (version 0.11.5)