FastQCFastQC Report
Thu 2 Jun 2016
DRR015158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16110450
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314580.19526456430453526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279280.1733533203603872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164270.10196487373102552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA325000.058.0572741
GTATCAA612150.041.608771
GTGGTAT124500.032.7321051
TGGTATC124200.031.9527872
TATCAAC800650.031.6497572
ATCAACG803200.031.426583
TCAACGC815500.030.9367084
CAACGCA859350.029.3689655
AACGCAG904050.027.9248226
GTACATG902050.026.7323781
TACATGG892500.026.24582
CGCAGAG1025400.024.5483978
ACGCAGA1037650.024.3069767
ACATGGG910300.024.2504983
GCAGAGT1096500.022.6038829
CATGGGG376700.022.2117424
CATGGGA417400.020.6127364
GAGTACT550350.020.14038312-13
CAGAGTA1049500.019.8119510-11
CATGGGT152650.019.671784
Produced by FastQC (version 0.11.5)