FastQCFastQC Report
Thu 2 Jun 2016
DRR015157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11295734
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276980.2452076155476041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231510.20495348066801147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144870.1282519577745014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231100.056.4411961
GTATCAA436600.041.4772721
GTGGTAT84100.033.822371
ATCAACG560350.032.1126483
TATCAAC565950.031.9077572
TCAACGC564950.031.8019964
TGGTATC91050.030.539642
CAACGCA592350.030.322735
AACGCAG619800.029.0454736
ACGCAGA704850.025.5008667
CGCAGAG710300.025.265988
GTACATG529650.025.0306151
TACATGG527500.024.337912
GCAGAGT755900.023.4347939
ACATGGG549600.022.4730453
CATGGGT153550.020.5978684
GAGTACT427900.020.33586712-13
CAGAGTA723450.020.24649210-11
AGAGTAC690600.019.6548310-11
CATGGGG288100.019.5711044
Produced by FastQC (version 0.11.5)