FastQCFastQC Report
Thu 2 Jun 2016
DRR015157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11295734
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322380.28539978012938333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283610.2510770880404939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167550.14833033426601583No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT117880.10435798151762427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA255100.057.5538181
GTATCAA479100.040.992921
GTGGTAT92450.034.9135671
TGGTATC97250.032.3837472
TATCAAC615550.031.7548752
ATCAACG614700.031.7374043
TCAACGC622500.031.339734
CAACGCA647400.030.1202985
AACGCAG676650.028.8818026
ACGCAGA771850.025.2722577
CGCAGAG774750.025.1316878
GTACATG530350.024.9881731
TACATGG526500.024.4710222
GCAGAGT817650.023.512759
ACATGGG547450.022.6714743
GAGTACT487200.020.6090612-13
CATGGGT155950.020.4509014
CAGAGTA787150.020.38652410-11
CATGGGG286550.019.846754
AGAGTAC751850.019.80660410-11
Produced by FastQC (version 0.11.5)