FastQCFastQC Report
Thu 2 Jun 2016
DRR015156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9550507
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197070.20634506628810387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171790.17987526735491632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102410.10722990936502114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187200.054.455531
GTATCAA339450.039.6035841
GTGGTAT74100.031.9363231
ATCAACG427450.031.0655653
TCAACGC431750.030.7769264
TATCAAC435000.030.6509552
TGGTATC76850.029.4883942
CAACGCA462450.028.7237935
AACGCAG488400.027.2251426
GTACATG389700.025.2946011
TACATGG385450.024.7450792
ACGCAGA538350.024.6979357
CGCAGAG536650.024.6221288
GCAGAGT574300.022.7306489
ACATGGG400150.022.6995373
CATGGGG213100.020.7566644
CAGAGTA545500.020.0615610-11
GAGTACT333500.019.82457512-13
AGAGTAC517150.019.00164810-11
GTACTTT336700.018.85807814-15
Produced by FastQC (version 0.11.5)