FastQCFastQC Report
Thu 2 Jun 2016
DRR015156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9550507
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224440.2350032307185367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202990.2125436900889136No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT154230.1614888089187307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117700.12323953063434225No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG109160.1142975969757417TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188850.056.5714071
GTATCAA357250.040.8882371
TGGTATC71450.032.5476382
GTGGTAT74050.032.4571041
ATCAACG456950.031.7518023
TATCAAC460200.031.6053772
TCAACGC463850.031.2794764
CAACGCA487850.029.7593215
AACGCAG514150.028.2955976
CGCAGAG579950.024.9841778
ACGCAGA583400.024.8984227
GTACATG393700.024.29191
TACATGG389000.023.8426972
GCAGAGT617100.023.2144669
ACATGGG405100.021.9117763
CAGAGTA593100.019.99852410-11
GAGTACT370050.019.6324812-13
CATGGGG218600.019.522494
AGAGTAC564700.019.49765410-11
AGTACTT364650.019.16821712-13
Produced by FastQC (version 0.11.5)