FastQCFastQC Report
Thu 2 Jun 2016
DRR015155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14646294
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218540.14921180743743093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175110.11955925505796894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236900.053.4821971
GTATCAA429950.041.376411
TATCAAC552950.031.8256322
ATCAACG550950.031.801073
TCAACGC552200.031.728544
CAACGCA587950.029.7992025
AACGCAG612200.028.655796
GTGGTAT94650.027.9838471
GTACATG667400.026.8053761
TACATGG658900.026.2591922
ACGCAGA691250.025.331567
CGCAGAG706200.024.709848
ACATGGG678700.024.4272463
TGGTATC109650.023.3247182
GCAGAGT762100.022.6286989
CATGGGT178850.021.4498584
CATGGGG360800.020.6979734
CATGGGA195900.019.5132964
CAGAGTA728600.019.31598310-11
AGAGTAC688600.018.8590110-11
Produced by FastQC (version 0.11.5)