FastQCFastQC Report
Thu 2 Jun 2016
DRR015155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14646294
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237770.16234140868672992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202420.1382056102383306No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT152340.10401266013095191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239700.052.8522721
GTATCAA445050.041.5274931
ATCAACG559600.032.7903863
TCAACGC566750.032.368684
TATCAAC567850.032.3544352
CAACGCA595400.030.8263135
AACGCAG621300.029.5731326
GTGGTAT102200.026.947781
GTACATG669300.026.9471051
TACATGG661200.026.4237212
ACGCAGA717450.025.5596477
CGCAGAG736500.024.8628278
ACATGGG687800.024.495573
TGGTATC112150.023.5768382
GCAGAGT789100.022.9058769
CATGGGT187050.021.8187924
CATGGGG366250.021.4416944
CAGAGTA756550.019.37476710-11
AGAGTAC717200.018.83587810-11
GAGTACT411050.018.77112612-13
Produced by FastQC (version 0.11.5)