FastQCFastQC Report
Thu 2 Jun 2016
DRR015154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8964932
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238530.26607006054256743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202970.22640439436685073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119410.13319677159849064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204350.059.057731
GTATCAA394750.040.8323441
GTGGTAT75500.036.8877641
TGGTATC75150.035.7871132
ATCAACG500100.031.8570733
TATCAAC504100.031.7034112
TCAACGC508100.031.3740984
CAACGCA549750.029.030095
AACGCAG566050.028.2100626
ACGCAGA624150.025.5612097
CGCAGAG629150.025.2917048
GTACATG479000.024.4350551
TACATGG473800.024.0015562
GCAGAGT660250.023.7213339
CATGGGT109100.023.0247344
ACATGGG482950.022.2937533
CAGAGTA641700.020.56380710-11
GTACTTT359200.020.11501114-15
GAGTACT374550.019.87433212-13
AATGCGG14550.019.3904197
Produced by FastQC (version 0.11.5)