FastQCFastQC Report
Thu 2 Jun 2016
DRR015154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8964932
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266020.2967339852661459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242360.27034226249568877No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT160430.1789528353366205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137680.15357617882656555No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG120420.1343233836017942RNA PCR Primer, Index 23 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT100110.11166844321853193RNA PCR Primer, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217350.058.559271
GTATCAA416750.040.7319221
GTGGTAT75300.038.690391
TGGTATC76450.036.607522
ATCAACG526150.032.1753653
TATCAAC533450.031.6740742
TCAACGC535550.031.6276114
CAACGCA568050.029.818095
AACGCAG592100.028.6037466
ACGCAGA659500.025.5984237
CGCAGAG659400.025.5482528
GTACATG485600.024.56071
TACATGG478750.024.295442
GCAGAGT697800.023.8100919
CATGGGT110500.022.5215594
ACATGGG492450.022.45933
CAGAGTA677350.020.62116410-11
GAGTACT400900.020.36960412-13
GTACTTT391500.020.18732514-15
AGTACTT391600.019.49954212-13
Produced by FastQC (version 0.11.5)