FastQCFastQC Report
Thu 2 Jun 2016
DRR015153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792375
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232460.23738878464111107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209950.21440151138002783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119540.12207457332873792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200850.056.0784531
GTATCAA375900.041.077521
ATCAACG477100.032.1061673
GTGGTAT79000.031.855961
TCAACGC481400.031.7817364
TGGTATC76550.031.7451692
TATCAAC487500.031.5424292
CAACGCA512900.029.8116465
AACGCAG535550.028.5673776
GTACATG393000.025.9155351
ACGCAGA591900.025.815777
CGCAGAG599000.025.417318
TACATGG392900.025.0991172
GCAGAGT632400.023.6503939
ACATGGG405450.023.2111683
CATGGGT115750.020.7979564
GAGTACT382350.020.63465512-13
CAGAGTA604150.020.34295810-11
CATGGGG204250.020.0746864
AGTACTT382250.019.73567412-13
Produced by FastQC (version 0.11.5)