FastQCFastQC Report
Thu 2 Jun 2016
DRR015153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792375
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267290.272957275431139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247740.2529927622257113No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT157330.16066582417442143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137330.14024176974431637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227750.057.086331
GTATCAA406750.038.925511
GTGGTAT85750.034.7727851
TGGTATC84100.034.1993642
ATCAACG517100.030.531153
TATCAAC518950.030.421162
TCAACGC525950.030.0087644
CAACGCA553900.028.5273655
AACGCAG579400.027.275526
GTACATG410000.024.4936751
ACGCAGA644650.024.4657337
CGCAGAG649550.024.1494628
TACATGG405750.023.8901042
GCAGAGT690900.022.4539099
ACATGGG421300.022.1278273
CATGGGT120800.020.9786014
GAGTACT427500.020.58193412-13
CAGAGTA665450.020.19066810-11
GTACTTT446900.019.24836714-15
CATGGGG208350.019.0419434
Produced by FastQC (version 0.11.5)