FastQCFastQC Report
Thu 2 Jun 2016
DRR015152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10070086
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243860.2421627779544286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212250.21077277790874874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128310.1274169853167093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204150.057.000591
GTATCAA379300.040.4818421
GTGGTAT79300.032.826221
TGGTATC80350.031.3761982
TATCAAC489900.031.24822
ATCAACG488450.031.1740363
TCAACGC492700.030.923454
CAACGCA527000.028.8588335
AACGCAG549150.027.7772626
GTACATG446550.025.0806941
ACGCAGA611200.024.8743827
CGCAGAG614700.024.7089468
TACATGG442500.024.5274012
GCAGAGT651350.023.0391969
ACATGGG452950.022.5856863
CATGGGT124450.021.976814
CATGGGG237250.020.5048474
GAGTACT376900.020.30436912-13
CAGAGTA625800.020.0604510-11
AGTACTT371250.019.51685712-13
Produced by FastQC (version 0.11.5)