FastQCFastQC Report
Thu 2 Jun 2016
DRR015152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10070086
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271080.2691933316160358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247750.2460257042491991No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT171890.17069367630028184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142760.1417664158975405No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG122990.12213401156653478RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT102310.10159794067299921RNA PCR Primer, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212150.057.9825941
GTATCAA393350.040.218971
GTGGTAT77850.034.209961
TGGTATC78250.033.1528362
ATCAACG505300.031.163683
TATCAAC506700.031.0422362
TCAACGC510850.030.8340174
CAACGCA538850.029.248695
AACGCAG565200.027.9852986
ACGCAGA638400.024.7329657
CGCAGAG643100.024.4619548
GTACATG446400.024.2619421
TACATGG441400.023.9007342
GCAGAGT682600.022.6999639
CATGGGT127000.022.1043554
ACATGGG460100.021.8938333
GAGTACT404900.020.1739412-13
CAGAGTA657300.020.11192510-11
CATGGGG236350.019.6354544
GTACTTT411550.019.18065814-15
Produced by FastQC (version 0.11.5)