FastQCFastQC Report
Thu 2 Jun 2016
DRR015151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12085487
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277580.2296804423355054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233110.19288424206653815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145240.12017720096840119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248250.058.043161
GTATCAA472200.041.416061
ATCAACG602550.032.1752933
GTGGTAT89850.032.1144561
TATCAAC608950.031.9863382
TCAACGC608900.031.8472214
CAACGCA638350.030.4061055
AACGCAG659950.029.431246
TGGTATC95750.028.8029042
GTACATG627250.028.341381
TACATGG623850.027.8521142
ACATGGG646800.025.7242183
ACGCAGA754900.025.6796077
CGCAGAG766850.025.2542368
CATGGGT239000.025.001894
GCAGAGT813750.023.5526839
CATGGGG299800.022.8308584
ATGGGTA97300.021.3281235
CAGAGTA781150.020.37826210-11
GAGTACT453100.020.16271812-13
Produced by FastQC (version 0.11.5)