FastQCFastQC Report
Thu 2 Jun 2016
DRR015151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12085487
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310480.25690317651245664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274340.2269995408542494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161010.13322590972130458No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT141060.11671850708208946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263700.057.883821
GTATCAA497400.041.8990141
GTGGTAT94700.033.4574661
ATCAACG638150.032.6036223
TATCAAC647300.032.1366732
TCAACGC647950.032.0894434
CAACGCA676400.030.7464565
TGGTATC100300.030.3996942
AACGCAG699550.029.7711756
GTACATG634550.027.3620471
TACATGG630050.026.999742
ACGCAGA803950.025.8653017
CGCAGAG816750.025.4055068
ACATGGG656100.024.8880543
CATGGGT244150.023.9274144
GCAGAGT870950.023.6002799
CATGGGG300500.022.0749914
ATGGGTA98600.020.395435
GAGTACT505000.020.39279712-13
CAGAGTA840350.020.27719510-11
Produced by FastQC (version 0.11.5)