FastQCFastQC Report
Thu 2 Jun 2016
DRR015150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847374
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254770.16076480557599007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217820.1374486397557097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA261250.054.7335361
GTATCAA494450.042.062731
TCAACGC628000.032.6816564
ATCAACG629800.032.660713
TATCAAC632000.032.6477282
CAACGCA660450.031.0826935
AACGCAG686050.029.9619796
GTGGTAT108250.028.4198911
GTACATG719350.026.6219041
TGGTATC111100.026.092392
ACGCAGA789950.025.9968857
TACATGG710750.025.931442
CGCAGAG810450.025.2479538
ACATGGG735100.024.0448863
GCAGAGT866300.023.2893249
CATGGGG430200.020.7017754
CATGGGT143350.020.0634924
CAGAGTA822250.019.76119410-11
GAGTACA550150.019.5803621
AGAGTAC783350.019.23828910-11
Produced by FastQC (version 0.11.5)