FastQCFastQC Report
Thu 2 Jun 2016
DRR015150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847374
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300740.18977276613778407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261080.16474653781755894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA275050.054.704291
GTATCAA520950.042.2359541
ATCAACG662050.033.0133823
TATCAAC662900.032.974282
TCAACGC664950.032.8830834
CAACGCA695100.031.4959535
AACGCAG721600.030.4485576
GTGGTAT109050.029.6410961
TGGTATC116150.026.8791582
ACGCAGA828500.026.4820637
GTACATG723850.025.8056341
CGCAGAG852100.025.669668
TACATGG712650.025.3846742
GCAGAGT907500.023.8019819
ACATGGG744650.023.339363
GAGTACA557550.020.1930471
CATGGGG436950.020.147774
CAGAGTA871500.019.93012410-11
AGAGTAC833650.019.5606210-11
GAGTACT511800.019.50821912-13
Produced by FastQC (version 0.11.5)