FastQCFastQC Report
Thu 2 Jun 2016
DRR015149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12945858
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243440.18804470124730244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203760.15739397110643422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238300.054.141921
GTATCAA443350.040.9264561
GTGGTAT97150.032.5571671
ATCAACG563350.031.9245513
TCAACGC571050.031.5654374
TATCAAC577850.031.3122332
TGGTATC101350.029.6348652
CAACGCA615400.029.2753725
AACGCAG644950.027.9834386
GTACATG694300.027.2809521
TACATGG687700.026.9127392
ACGCAGA714600.025.2357947
CGCAGAG728500.024.7281538
ACATGGG722350.024.5320823
GCAGAGT782950.022.7236739
CATGGGG330200.021.6924574
CATGGGA301800.020.793434
GAGTACA527250.019.4209521
CAGAGTA759550.019.31103710-11
AGTACAT508350.018.8887142
Produced by FastQC (version 0.11.5)