FastQCFastQC Report
Thu 2 Jun 2016
DRR015149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12945858
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281070.21711191332393726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240490.1857659801304788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142380.10998112291977866No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT140600.10860616577132239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248700.056.5437551
GTATCAA471300.041.322431
ATCAACG597650.032.5590253
TCAACGC608100.032.014474
GTGGTAT91450.032.0088271
TATCAAC607400.031.9415152
CAACGCA644700.030.2180445
TGGTATC96850.029.2284452
AACGCAG673250.028.9196326
GTACATG698300.026.7421361
TACATGG697150.026.269192
ACGCAGA766000.025.3883427
CGCAGAG775500.025.072928
ACATGGG732000.024.0846123
GCAGAGT834250.023.0672789
CATGGGG332350.021.793474
CATGGGA301650.020.9949534
CAGAGTA813750.019.58656710-11
GAGTACA530050.019.5392131
GAGTACT455400.019.0261212-13
Produced by FastQC (version 0.11.5)