FastQCFastQC Report
Thu 2 Jun 2016
DRR015148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13319307
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297080.22304463738241037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244190.1833353642197751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155130.1164700235530272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256750.055.888471
GTATCAA487000.041.7628481
GTGGTAT94650.034.619481
ATCAACG630100.031.9377943
TCAACGC632700.031.7702264
TATCAAC636250.031.700252
TGGTATC101500.031.0711942
CAACGCA673750.029.8884645
AACGCAG702500.028.7232486
GTACATG755750.027.7788331
TACATGG747450.027.3007262
ACATGGG766550.025.2731173
ACGCAGA801000.025.1218057
CGCAGAG800350.025.0716238
CATGGGT294800.024.6837834
GCAGAGT864100.022.979719
CATGGGG339300.021.2989124
GAGTACA559150.021.2269671
AGTACAT543850.020.3505042
ATGGGTG171850.020.3111695
Produced by FastQC (version 0.11.5)