FastQCFastQC Report
Thu 2 Jun 2016
DRR015148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13319307
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344230.2584443770235193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297090.2230521452805315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175490.13175610412763966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA275500.057.5023161
GTATCAA533250.042.624411
GTGGTAT102250.035.216071
ATCAACG685550.033.12943
TGGTATC107150.032.746332
TATCAAC695150.032.65492
TCAACGC697700.032.5785564
CAACGCA734100.030.9507685
AACGCAG769400.029.5790826
GTACATG751750.026.6262951
TACATGG749100.026.1537382
ACGCAGA878850.025.8598187
CGCAGAG878550.025.8024988
ACATGGG767900.024.4397873
CATGGGT294600.024.2771634
GCAGAGT942450.023.8070379
CATGGGG342800.021.1556134
GAGTACT506650.020.84256212-13
GAGTACA568950.020.0669381
CAGAGTA916700.020.01702310-11
Produced by FastQC (version 0.11.5)