FastQCFastQC Report
Thu 2 Jun 2016
DRR015147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22833655
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532220.23308576747787418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457830.20050666439516582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271740.11900854243440219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA425000.057.2125551
GTATCAA821650.042.585751
GTGGTAT157000.033.1853751
ATCAACG1050450.032.998213
TCAACGC1060650.032.7047734
TATCAAC1072950.032.463012
CAACGCA1128650.030.7364225
TGGTATC168350.029.6224732
AACGCAG1187950.029.2668276
ACGCAGA1323100.026.243097
CGCAGAG1326150.026.110228
GTACATG1006050.025.6131941
TACATGG986000.025.1779062
GCAGAGT1409000.024.2550549
ACATGGG1031550.023.087583
CATGGGT265850.021.1530274
CAGAGTA1351800.020.6109410-11
GAGTACT822100.020.54495412-13
CATGGGG537150.020.1500364
AGAGTAC1288600.019.7877810-11
Produced by FastQC (version 0.11.5)