FastQCFastQC Report
Thu 2 Jun 2016
DRR015147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22833655
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595500.2607992456748602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT539410.23623462822750016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321670.14087538766789637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA467850.057.3913461
GTATCAA891550.041.7774121
GTGGTAT167550.035.669331
TGGTATC179200.032.1998672
ATCAACG1162000.031.7980943
TCAACGC1175400.031.480944
TATCAAC1180900.031.406342
CAACGCA1231600.030.011565
AACGCAG1301700.028.4626966
ACGCAGA1476900.025.0680487
CGCAGAG1476350.024.9911588
GTACATG1028500.024.9779851
TACATGG1013900.024.6046262
GCAGAGT1558500.023.3792279
ACATGGG1043350.022.6544343
CATGGGT272350.021.4804234
CAGAGTA1500450.020.22128710-11
GAGTACT942000.020.16445412-13
AGAGTAC1437150.019.79660210-11
AGTACTT932150.019.47446412-13
Produced by FastQC (version 0.11.5)