FastQCFastQC Report
Thu 2 Jun 2016
DRR015146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22946445
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474190.2066507469893485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405470.17670275286651155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240000.10459136480618239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA405650.057.5985531
GTATCAA802450.041.1245651
GTGGTAT157600.033.516821
TGGTATC155000.032.874452
ATCAACG1001400.032.477673
TCAACGC1012600.032.094984
TATCAAC1029000.031.7605232
CAACGCA1113300.029.2193475
AACGCAG1165400.027.9931556
GTACATG973350.026.4232771
TACATGG956600.025.9005172
ACGCAGA1268850.025.6728157
CGCAGAG1276900.025.4431048
GCAGAGT1344900.023.6084429
ACATGGG995700.023.5911313
CATGGGG513600.020.4158464
CATGGGA352050.020.2399044
CAGAGTA1309550.019.97156510-11
CATGGGT182300.019.3059584
GAGTACT825950.019.19380612-13
Produced by FastQC (version 0.11.5)