FastQCFastQC Report
Thu 2 Jun 2016
DRR015146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22946445
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462020.20134709319896826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423140.18440329210036674No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT353230.15393669912703253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238920.10412070366455457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA422150.058.616851
GTATCAA806700.039.7859951
TGGTATC155200.033.130622
GTGGTAT161950.032.7678221
ATCAACG1011900.031.162893
TCAACGC1023600.030.8115484
TATCAAC1037500.030.560672
CAACGCA1103500.028.5604975
AACGCAG1156750.027.2935246
GTACATG1007650.026.00711
TACATGG985800.025.691962
ACGCAGA1297050.024.299617
CGCAGAG1301300.024.1572178
ACATGGG1032900.023.3539773
GCAGAGT1374250.022.5306849
CATGGGG532650.020.9155584
CATGGGA360650.019.9419924
CAGAGTA1332450.019.64299610-11
AGTACAT746450.018.9584352
GAGTACA798200.018.9530561
Produced by FastQC (version 0.11.5)