FastQCFastQC Report
Thu 2 Jun 2016
DRR015145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28052082
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443350.15804531014845885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372670.13284931934820382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA506250.053.4666821
GTATCAA925750.041.2309571
ATCAACG1182500.031.8401833
GTGGTAT187400.031.6624341
TCAACGC1193900.031.5576884
TATCAAC1210700.031.2861292
CAACGCA1298900.028.9595745
AACGCAG1340950.028.061396
TGGTATC214300.026.859282
GTACATG1586900.025.8980541
TACATGG1593050.025.3893072
ACGCAGA1497800.025.077987
CGCAGAG1557850.024.09618
ACATGGG1644100.023.396423
GCAGAGT1638850.022.5413289
CATGGGA766950.021.5973634
CATGGGG788650.020.391144
CAGAGTA1579850.019.20963910-11
GAGTACA1228500.018.8636931
AGTACAT1155000.018.8064082
Produced by FastQC (version 0.11.5)