FastQCFastQC Report
Thu 2 Jun 2016
DRR015145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28052082
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491980.1753809218153576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440450.15701151878851632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA526200.055.6028941
GTATCAA977250.041.1503261
GTGGTAT194250.033.226731
ATCAACG1252900.031.6616023
TCAACGC1265250.031.3593734
TATCAAC1280700.031.1435742
CAACGCA1356350.029.246925
TGGTATC217350.028.6830182
AACGCAG1395650.028.4502936
GTACATG1593000.024.9757041
ACGCAGA1588000.024.9301937
TACATGG1595050.024.4866142
CGCAGAG1647550.024.0346268
ACATGGG1636450.022.5448423
GCAGAGT1738750.022.4548829
CATGGGA755100.020.8629474
CATGGGG786350.019.6173154
CAGAGTA1674900.019.45188110-11
GTACTTT798300.018.77060114-15
AGAGTAC1605750.018.41955210-11
Produced by FastQC (version 0.11.5)