FastQCFastQC Report
Thu 2 Jun 2016
DRR015144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27607997
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550260.1993118153410405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452830.16402131599767997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280790.10170603829028234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA495900.055.294761
GTATCAA924750.042.1805731
ATCAACG1192650.032.385623
TCAACGC1199150.032.22834
TATCAAC1208950.032.170592
GTGGTAT185950.030.4182971
CAACGCA1271500.030.3994795
AACGCAG1332700.029.0894136
TGGTATC199500.027.1195832
GTACATG1335050.026.7816261
TACATGG1325350.026.1907442
ACGCAGA1497950.025.8261727
CGCAGAG1533050.025.1923568
ACATGGG1367100.024.1393133
GCAGAGT1633250.023.209349
CATGGGT426850.023.0854684
CATGGGG681850.020.2486954
CAGAGTA1574550.019.75072710-11
GAGTACA1037250.019.5691
AGAGTAC1492500.019.28810710-11
Produced by FastQC (version 0.11.5)