FastQCFastQC Report
Thu 2 Jun 2016
DRR015144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27607997
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612880.22199364915897377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529240.1916980793644682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321050.11628876951848409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA514500.056.1325721
GTATCAA976500.042.2303471
GTGGTAT191350.032.5406571
ATCAACG1272350.032.0725143
TCAACGC1279000.031.9182224
TATCAAC1287850.031.8292472
CAACGCA1340650.030.484725
AACGCAG1408200.029.0751386
TGGTATC207200.028.8142492
GTACATG1340950.026.0346341
ACGCAGA1607200.025.4414757
TACATGG1332550.025.4069562
CGCAGAG1641050.024.8640658
ACATGGG1375250.023.4993443
GCAGAGT1743850.023.095759
CATGGGT437300.022.3240724
GAGTACT980300.019.6552812-13
GAGTACA1051700.019.627011
CAGAGTA1690350.019.55610810-11
CATGGGG685300.019.48944
Produced by FastQC (version 0.11.5)