FastQCFastQC Report
Thu 2 Jun 2016
DRR015143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22693086
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477080.21023143348595252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426640.1880043992253852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247980.10927557406692065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA430400.056.6487771
GTATCAA804150.040.365941
GTGGTAT166900.032.060181
ATCAACG1018800.031.522633
TATCAAC1034750.031.1632442
TCAACGC1034500.031.0827474
TGGTATC173050.029.7891652
CAACGCA1107100.029.0592255
AACGCAG1137650.028.292356
GTACATG994000.025.9352951
TACATGG974500.025.3840792
ACGCAGA1276500.025.1728367
CGCAGAG1293950.024.81988
ACATGGG1012600.023.2977053
GCAGAGT1389250.022.71429
CATGGGT273400.021.2674064
CATGGGG513650.020.1579024
CAGAGTA1332900.020.05529210-11
GAGTACT790950.019.71811912-13
AGAGTAC1260500.019.07783710-11
Produced by FastQC (version 0.11.5)