FastQCFastQC Report
Thu 2 Jun 2016
DRR015143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22693086
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517960.22824573088032185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477140.21025787325707926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271420.11960471132044359No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT250450.11036401131163914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA445800.057.6865961
GTATCAA827800.040.5566441
GTGGTAT171950.033.9882931
TGGTATC176800.031.7104992
ATCAACG1058100.031.3551833
TCAACGC1073300.030.9449214
TATCAAC1085200.030.7584842
CAACGCA1137350.029.1903825
AACGCAG1180000.028.1358226
GTACATG1001550.025.690521
TACATGG987950.025.2233682
ACGCAGA1336000.024.8099677
CGCAGAG1355600.024.421058
ACATGGG1014950.023.1803133
GCAGAGT1445050.022.6166789
CATGGGT274100.021.4594024
CAGAGTA1387850.020.0143210-11
GAGTACT855450.020.01050812-13
CATGGGG519400.019.5575714
GTACTTT861700.019.28793114-15
Produced by FastQC (version 0.11.5)