FastQCFastQC Report
Thu 2 Jun 2016
DRR015142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30486984
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559880.1836455846206368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468450.15365573714999162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA533900.054.5395431
GTATCAA991850.041.970731
ATCAACG1291900.031.88183
TCAACGC1301250.031.6827014
TATCAAC1326550.031.1957192
CAACGCA1384900.029.7601455
GTGGTAT213900.029.6791041
AACGCAG1435700.028.7588026
GTACATG1618600.027.8713251
TGGTATC220100.027.6429582
TACATGG1612250.027.2551142
CATGGGT574450.025.7061584
ACATGGG1656300.025.483383
ACGCAGA1637450.025.1374667
CGCAGAG1664350.024.7081158
GCAGAGT1783400.022.6733449
CATGGGG777700.021.9095824
GAGTACA1206300.020.8996751
ATGGGTA274700.020.602575
AGTACAT1164550.020.483562
Produced by FastQC (version 0.11.5)