FastQCFastQC Report
Thu 2 Jun 2016
DRR015142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30486984
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629850.20659636256574282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554220.1817890546339382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331560.10875460819607477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA584950.056.296911
GTATCAA1085700.041.9316331
GTGGTAT216050.032.6755031
ATCAACG1417450.031.879123
TCAACGC1426550.031.6688334
TATCAAC1442450.031.4430852
TGGTATC225900.030.2965372
CAACGCA1498150.030.1797455
AACGCAG1556550.029.0777116
GTACATG1635650.026.5947551
TACATGG1626400.026.0037962
ACGCAGA1799200.025.0933727
CGCAGAG1822250.024.7510768
CATGGGT578450.024.4107954
ACATGGG1676750.024.1896933
GCAGAGT1948850.022.8796129
CATGGGG803550.020.2729724
ATGGGTA272400.020.1905145
GAGTACA1243400.020.0528431
GAGTACT1055850.019.72763812-13
Produced by FastQC (version 0.11.5)