FastQCFastQC Report
Thu 2 Jun 2016
DRR015141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19774271
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450890.2280185196207739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375260.18977185050209944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228920.11576659387342268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA352350.055.7674261
GTATCAA677350.042.3821071
ATCAACG870550.032.629983
TCAACGC876400.032.3618244
TATCAAC884150.032.3347972
CAACGCA923850.030.7041475
GTGGTAT140200.030.29051
AACGCAG953650.029.8041636
TGGTATC153950.026.402142
ACGCAGA1079450.026.273997
GTACATG838150.025.6271931
CGCAGAG1107750.025.5926678
TACATGG826850.025.0633012
GCAGAGT1185500.023.5859939
ACATGGG856800.023.0763283
CAGAGTA1151400.019.72466710-11
CATGGGG458550.019.6113824
GAGTACA673250.019.5453991
AGAGTAC1094750.019.35347410-11
CATGGGT204500.019.3390184
Produced by FastQC (version 0.11.5)