FastQCFastQC Report
Thu 2 Jun 2016
DRR015141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19774271
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487460.2465122481632825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419960.21237698219064563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255040.12897567753572306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA384850.057.204171
GTATCAA729150.042.1861531
ATCAACG944500.032.395263
TCAACGC953750.032.0981254
TATCAAC955900.031.9911082
GTGGTAT139650.031.5549351
CAACGCA996500.030.7165475
AACGCAG1032050.029.6800526
TGGTATC153200.027.7434542
ACGCAGA1173300.026.0490727
GTACATG852450.025.6226621
CGCAGAG1201000.025.3997428
TACATGG839400.025.1439912
GCAGAGT1272950.023.6318119
ACATGGG872300.023.325293
CATGGGG467150.020.2665334
CAGAGTA1232100.020.18136610-11
CATGGGT208250.019.9675544
GAGTACT791000.019.73689812-13
AGAGTAC1172950.019.7175510-11
Produced by FastQC (version 0.11.5)