FastQCFastQC Report
Thu 2 Jun 2016
DRR015140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17377793
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459170.2642280294166239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388340.2234691137131165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232070.13354400066797895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA340750.057.50241
GTATCAA655650.041.8317221
GTGGTAT120250.035.0867651
ATCAACG836100.032.3935933
TATCAAC843900.032.3435752
TCAACGC839700.032.1431664
TGGTATC132050.031.0549832
CAACGCA892050.030.2310715
AACGCAG921150.029.3485416
GTACATG826150.026.3836351
ACGCAGA1036150.026.033717
TACATGG825650.025.7433952
CGCAGAG1062150.025.3511248
GCAGAGT1126250.023.5748189
ACATGGG859150.023.56263
CATGGGG449550.020.7824064
CATGGGT214650.020.670864
CAGAGTA1088350.020.34427810-11
GAGTACT668750.019.73647312-13
GAGTACA637600.019.6805441
Produced by FastQC (version 0.11.5)